Usage
KOD One series enables fast PCR, which has an extension time of 5 sec/kb by applying UKOD a new Elongation Accelerator. These master mixes provide greater efficiency and elongation capabilities than conventional PCR enzymes. These show greater amplification success from crude specimens.
KOD One series contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and 3’→5’ exonuclease activities, thus allowing for Hot Start PCR. Primers or Templates Containing Inosines (dI) or Uracils (dU) can be used. KOD One series can use primers or templates containing Inosines (dI) or Uracils (dU), whereas conventional high-fidelity PCR enzymes cannot.
Key Specifications
PCR Assay 10kbp fragment could be amplified from human genomic DNA using fast cycle. Extension time is 50 seconds.
Key Characteristics
Fast
High fidelity and high efficiency
Amplification from crude samples
Applicable for Direct PCR, Colony PCR, Amplification of NGS libraries and site directed gene mutation
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