TTx DNA Polymerase

TTx DNA Polymerase

Origin: Escherichia coli, Recombinant

Usage
Effective for amplification from crude samples containing PCR inhibitors with high efficiency for both DNA and RNA. Suitable for amplification from low copies of template DNA/RNA and is efficient even in fast cycle condition.
In addition, TTx DNA Polymerase has a 5 ‘- 3’ exonuclease activity, so it can be used for real-time PCR using probe assays such as TaqMan® assay.

Key Specifications

Enzyme Specification

• Ribonuclease Activity: None detected
• Endonuclease Activity: None detected
• Nicking Activity: None detected

Hot Start TTx [DNA/RNA] Kit Specification

• Tested for functional performance by amplifying from dilutions of enterovirus RNA in combination with 5x Buffer for rTth/ TTx (DNA/ RNA)
• The slope from analysis is as follows: -3.74 ≤ slope ≤ -3.01
• The correlation coefficients from both analyses are as follows: r ≤ -0.980

Key Characteristics

• Enables highly efficient 1-enzyme 1-step RT-PCR
• Effective amplification from crude samples
• Fast- High speed qPCR cycling detection
• Suitable for Multiplex PCR