Usage
- TTx DNA Polymerase exhibits reverse transcription activity in the presence of Mn2+ ions and allows for 1-Step 1-Enzyme RT-PCR, including reverse transcription and PCR steps. Effective for amplification from crude samples containing PCR inhibitors with high efficiency for both DNA and RNA. Suitable for amplification from low copies of template DNA/RNA and is efficient even in fast cycle condition.
- In addition, TTx DNA Polymerase has a 5 ‘- 3’ exonuclease activity, so it can be used for real-time PCR using probe assays such as TaqMan® assay.
Key Specifications
Enzyme Specification
- Ribonuclease Activity: None detected
- Endonuclease Activity: None detected
- Nicking Activity: None detected
Hot Start TTx [DNA/RNA] Kit Specification
- Tested for functional performance by amplifying from dilutions of enterovirus RNA in combination with 5x Buffer for rTth/ TTx (DNA/ RNA)
- The slope from analysis is as follows: -3.74 ≤ slope ≤ -3.01
- The correlation coefficients from both analyses are as follows: r ≤ -0.980
Key Characteristics
- Enables highly efficient 1-enzyme 1-step RT-PCR
- Effective amplification from crude samples
- Fast- High speed qPCR cycling detection
- Suitable for Multiplex PCR
Product Availability: US, Europe, Asia (only in selected countries)
Origin: Escherichia coli, Recombinant
Safety Data Sheets
Contact us to get more information, become part of our distribution network, or ask a question about our products and their availability in your region:
Name | Part | Size |
---|---|---|
TTx DNA Polymerase | TTX-119 | TTx DNA Polymerase 1KU |
TTx DNA Polymerase | TTX-129 | TTx DNA Polymerase 10KU |
TTx DNA Polymerase | TTX-159 | TTx DNA Polymerase 100KU |
TTx DNA Polymerase (Glycerol free) | TTX-219 | TTx DNA Polymerase (Glycerol free) 1KU |
TTx DNA Polymerase (Glycerol free) | TTX-229 | TTx DNA Polymerase (Glycerol free) 10KU |
TTx DNA Polymerase (Glycerol Free) | TTX-259 | TTx DNA Polymerase (Glycerol free) 100KU |
Hot Start TTx [DNA] kit | HSTTX-101 | Hot Start TTx DNA Polymerase (4U/uL) 62.5uL; 2x Buffer for rTth/TTx (DNA) 1.25mL x2 |
Hot Start TTx [RNA] kit | HSTTX-111 | Hot Start TTx DNA Polymerase (4U/uL) 62.5uL; 5x Buffer for rTth/TTx (DNA/RNA) 1mL; 50mM Mn (OAc)2 250uL; 2mM dNTPs 1 mL |
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