TTx DNA Polymerase

Usage

• TTx DNA Polymerase exhibits reverse transcription activity in the presence of Mn2+ ions and allows for 1-Step 1-Enzyme RT-PCR, including reverse transcription and PCR steps. Effective for amplification from crude samples containing PCR inhibitors with high efficiency for both DNA and RNA. Suitable for amplification from low copies of template DNA/RNA and is efficient even in fast cycle condition.
• In addition, TTx DNA Polymerase has a 5 ‘- 3’ exonuclease activity, so it can be used for real-time PCR using probe assays such as TaqMan® assay.

Key Specifications

Enzyme Specification

• Ribonuclease Activity: None detected
• Endonuclease Activity: None detected
• Nicking Activity: None detected

Hot Start TTx [DNA/RNA] Kit Specification

• Tested for functional performance by amplifying from dilutions of enterovirus RNA in combination with 5x Buffer for rTth/ TTx (DNA/ RNA)
• The slope from analysis is as follows: -3.74 ≤ slope ≤ -3.01
• The correlation coefficients from both analyses are as follows: r ≤ -0.980

 Key Characteristics

• Enables highly efficient 1-enzyme 1-step RT-PCR
• Effective amplification from crude samples
• Fast- High speed qPCR cycling detection
• Suitable for Multiplex PCR

Product Availability: US, Europe, Asia (only in selected countries)

Origin: Escherichia coli, Recombinant