Products » Enzymes


Taq DNA Polymerase

EC Number

  • EC 2.7.7.7

Usage

  • Taq DNA polymerase is the most widely used thermostable DNA polymerase derived from the thermophilic bacteria Thermus aquaticus (Taq ) YT-1. The enzyme possesses a 5’→3’ polymerase activity and a double-strand specific 5’→3’ exonuclease activity.

Key Specifications

Enzyme Specification:

  • Concentration/Activity*: 5U/µL
  • Purity: ≥90%
  • Endonuclease Activity: None detected
  • Nicking Activity: None detected

*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 minutes at 75°C.

Hot Start Antibody Specification:

  • Source: Mouse Hybridoma
  • Concentration: 1mg/mL
  • Inhibition Activity**: ≥95%
  • Mouse Genomic DNA Contamination: None detected

**Percentage (%) of DNA polymerase activity suppressed when 1 µG is added to 5 units of Taq DNA Polymerase at 40°C

Key Characteristics

  • Tolerates various kinds of PCR protocols
  • Applicable for hot start technology by adding anti-Taq antibody
  • PCR products can be cloned by using a TA cloning method
  • Incorporates dUTP, dITP, and fluorescently-labelled nucleotides

Product Availability: US, Europe, Asia (only in selected countries)

Origin: Escherichia coli, Recombinant

Safety Data Sheets

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Name Part Size
Taq DNA Polymerase Kit A TAP-211 50μL (250U) Taq DNA Polymerase, 1mL 10x Buffer (+Mg) for Taq, 1mL 2mM dNTPs
Taq DNA Polymerase 10KU TAP-229E 2mL (10KU) Taq DNA Polymerase
Taq DNA Polymerase 100KU TAP-259E 20mL (100KU) Taq DNA Polymerase
10x Buffer (+Mg) for Taq TAP-2M 1 mL 10x Buffer (+Mg) for Taq
HS Antibody (Taq) Kit TCP-101 0.1mL (0.1mg) anti-Taq high, 1mL 10x PCR Buffer anti-Taq high
HS Antibody (Taq) 30mg TCP-139 30mL anti-Taq high
HS Antibody (Taq) 100mg TCP-189CH1 100mL anti-Taq high

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