EC Number
- EC 2.7.7.7
Usage
- Taq DNA polymerase is the most widely used thermostable DNA polymerase derived from the thermophilic bacteria Thermus aquaticus (Taq ) YT-1. The enzyme possesses a 5’→3’ polymerase activity and a double-strand specific 5’→3’ exonuclease activity.
Key Specifications
Enzyme Specification:
- Concentration/Activity*: 5U/µL
- Purity: ≥90%
- Endonuclease Activity: None detected
- Nicking Activity: None detected
*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 minutes at 75°C.
Hot Start Antibody Specification:
- Source: Mouse Hybridoma
- Concentration: 1mg/mL
- Inhibition Activity**: ≥95%
- Mouse Genomic DNA Contamination: None detected
**Percentage (%) of DNA polymerase activity suppressed when 1 µG is added to 5 units of Taq DNA Polymerase at 40°C
Key Characteristics
- Tolerates various kinds of PCR protocols
- Applicable for hot start technology by adding anti-Taq antibody
- PCR products can be cloned by using a TA cloning method
- Incorporates dUTP, dITP, and fluorescently-labelled nucleotides
Product Availability: US, Europe, Asia (only in selected countries)
Origin: Escherichia coli, Recombinant
Safety Data Sheets
Contact us to get more information, become part of our distribution network, or ask a question about our products and their availability in your region:
| Name | Part | Size |
|---|---|---|
| Taq DNA Polymerase Kit A | TAP-211 | 50μL (250U) Taq DNA Polymerase, 1mL 10x Buffer (+Mg) for Taq, 1mL 2mM dNTPs |
| Taq DNA Polymerase 10KU | TAP-229E | 2mL (10KU) Taq DNA Polymerase |
| Taq DNA Polymerase 100KU | TAP-259E | 20mL (100KU) Taq DNA Polymerase |
| 10x Buffer (+Mg) for Taq | TAP-2M | 1 mL 10x Buffer (+Mg) for Taq |
| HS Antibody (Taq) Kit | TCP-101 | 0.1mL (0.1mg) anti-Taq high, 1mL 10x PCR Buffer anti-Taq high |
| HS Antibody (Taq) 30mg | TCP-139 | 30mL anti-Taq high |
| HS Antibody (Taq) 100mg | TCP-189CH1 | 100mL anti-Taq high |
