RNase Inhibitor

EC Number

• EC 2.7.7.49

Usage

• This product is a recombinant type human ribonuclease inhibitor purified using a combination of ion exchange and affinity chromatography. This inhibitor exhibits broad-spectrum RNase inhibitory properties, including RNase A, RNase B, RNase C and human placental RNase. Does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, and RNase H. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. This inhibitor can be applied to a reverse transcriptase reaction.

Key Specifications

• Concentration/Activity**: 40 U/µl
• Purity: ≥90%
• RNase Activity: None detected
• Endonuclease Activity: None detected
• Exonuclease activity: None detected

** One unit is defined as the amount of RNase inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50 %.

Key Characteristics

• Stabilizes RNA in a cDNA synthesis or a RNA synthesis reaction
• Does not inhibit SP6, T7 or T3 RNA Polymerase, AMV or M-MLV Reverse Transcriptase, or Taq DNA Polymerase
• Exhibits broad-spectrum RNase inhibitory properties
• Active over a broad pH range (pH 5–8)

Product Availability: US, Europe, Asia (only in selected countries)

Origin: Escherichia coli, Recombinant