EC Number
- EC 2.7.7.49
Usage
- This product is a recombinant type human ribonuclease inhibitor purified using a combination of ion exchange and affinity chromatography. This inhibitor exhibits broad-spectrum RNase inhibitory properties, including RNase A, RNase B, RNase C and human placental RNase. Does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, and RNase H. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. This inhibitor can be applied to a reverse transcriptase reaction.
Key Specifications
- Concentration/Activity**: 40 U/µl
- Purity: ≥90%
- RNase Activity: None detected
- Endonuclease Activity: None detected
- Exonuclease activity: None detected
** One unit is defined as the amount of RNase inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50 %.
Key Characteristics
- Stabilizes RNA in a cDNA synthesis or a RNA synthesis reaction
- Does not inhibit SP6, T7 or T3 RNA Polymerase, AMV or M-MLV Reverse Transcriptase, or Taq DNA Polymerase
- Exhibits broad-spectrum RNase inhibitory properties
- Active over a broad pH range (pH 5–8)
Product Availability: US, Europe, Asia (only in selected countries)
Origin: Escherichia coli, Recombinant
Safety Data Sheets
Contact us to get more information, become part of our distribution network, or ask a question about our products and their availability in your region:
Name | Part | Size |
---|---|---|
RNase Inhibitor 2.5KU | SIN-201 | 62.5µL (2,500U) RNase Inhibitor |
RNase Inhibitor 100KU | SIN-229 | 2.5mL (100KU) RNase Inhibitor (Bulk) |
RNase Inhibitor 1,000KU | SIN-259 | 25mL (1,000KU) RNase Inhibitor (Bulk) |