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RNA-direct Realtime PCR Master Mix

Usage

  • This product is a 2 × Master Mix for “1-step real-time PCR” using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8. Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for “1-step real-time PCR”, including reverse transcription and PCR steps. RNA-direct Realtime PCR Master Mix is applicable for TaqMan assay or hybridization probe assay, in combination with each probe. RNA-direct SYBR Green Realtime PCR Master Mix can be applied to an intercalation assay with SYBR Green I.

Key Specifications

  • Concentration: 40 reactions / mL
  • Functional test*: Correlation coefficient |r | ≥0.98
  • Bacterial DNA contamination : Ct ≥ 27
  • Ribonuclease Activity : None detected

*Amplification of a G3PDH region from serial dilutions of human G3PDH control RNA and no-template control.

Key Characteristics

  • Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination
  • Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.)
  • Can be used in systems using passive reference (e.g., ABI PRISM 7000, 7700, 7900HT, StepOne Plus, Applied Biosystems, Inc.)
  • Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible amplification

Product Availability: US, Europe, Asia (only in selected countries)

Origin: Escherichia coli, Recombinant

Safety Data Sheets

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Name Part Size
RNA-direct Realtime PCR Master Mix Kit QRT-101 5x 0.5mL RNA-direct Realtime PCR Master Mix, 0.5mL 50mM Mn(OAc)2 (100 reactions)
RNA-direct Realtime PCR Master Mix (SYBR Green) Kit QRT-201 5x 0.5mL RNA-direct SYBR Green Realtime PCR Master Mix, 0.5mL 50mM Mn(OAc)2 (100 reactions)

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