Products » Enzymes


Reverse Transcriptase

EC Number

  • EC 2.7.7.49

Usage

  • ReverTra Ace® is a high efficient M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase that has been genetically modified to remove RNase H activity and increase reaction efficiency. It is the preferred enzyme for applications requiring full-length cDNAs and high product yields from total RNA, mRNA, rRNA, etc.

Key Specifications

  • Concentration/Activity*: 100 U/µL
  • Ribonuclease Activity: None detected

*One unit is defined as the amount of enzyme that will incorporate 1 nmole of dTTP into an acid insoluble material in 10 minutes at 42°C.

Key Characteristics

  • RNase minus M-MLV RTase with improved performance
  • Enables the synthesis of longer cDNAs (≥ 14 kb) than the WT-enzyme
  • Exhibits excellent reaction efficiency at high temperatures

Product Availability: US, Europe, Asia (only in selected countries)

Origin: Escherichia coli, Recombinant

Safety Data Sheets

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Name Part Size
ReverTra Ace Rtase Kit TRT-101 0.1mL (10,000U) Reverse Transcriptase, 1mL 5x RT Buffer
ReverTra Ace Rtase 50KU TRT-109 5mL (500 KU Reverse Transcriptase
5x Rtase Buffer 1mL TRT-1B 1mL 5x RT Buffer

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