KOD SYBR qPCR Mix

EC Number: EC 2.7.7.7

Usage

KOD SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using SYBR® Green I and based on the KOD exo(-) DNA Polymerase. KOD exo(-) DNA Polymerase is a 3’→5’ exonuclease minus mutant developed based on KOD DNA polymerase from a hyperthermophilic Archaeon Thermococcus kodakaraensis. The master mix contains all the required components, except the ROX reference dye and primers (50x ROX reference dye is supplied separately with this kit). The master mix aids reaction setup and improves the reproducibility of experiments. This product is optimized to be highly efficient and robust in the SYBR® Green assay.

Key Specifications

Concentration: 40 reactions/mL

Functional Test: -1.000 ≤ correlation coefficient ≤ -0.990
______________3.920 ≤ slope ≤ -3.103

Bacterial Contamination: Ct ≥ 28

*Functional test -Amplification of a 582bp human β-globin region from dilutions of human genomic DNA standard and no-template control

Key Characteristics

  • Effective for GC rich targets
  • Long target amplification (~2kb)
  • High specificity
  • Effective amplification from crude samples
  • Compatibility with various real-time cyclers
  • Can be used for library quantification

Product Availability: US, Europe, Asia (only in selected countries)

Origin: Escherichia coli, Recombinant

Safety Data Sheets

Contact us to get more information, become part of our distribution network, or ask a question about our products and their availability in your region:

Contact Us
Name Part Size
KOD SYBR qPCR Mix Kit QKD-201 3x 1.67mL KOD SYBR qPCR Mix
1 x 250µL 50x ROX reference dye
KOD SYBR qPCR Mix QKD-259 100mL KOD SYBR qPCR Mix

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