KOD SYBR qPCR Mix

KOD SYBR qPCR Mix

EC Number

2.7.7.7

Origin: Escherichia coli, Recombinant

Usage
KOD exo(-) DNA Polymerase is a 3’→5’ exonuclease minus mutant developed based on KOD DNA polymerase from a hyperthermophilic Archaeon Thermococcus kodakaraensis. The master mix contains all the required components, except the ROX reference dye and primers (50x ROX reference dye is supplied separately with this kit). The master mix aids reaction setup and improves the reproducibility of experiments. This product is optimized to be highly efficient and robust in the SYBR® Green assay.

Key Specifications

Concentration: 40 reactions/mL

Functional Test: -1.000 ≤ correlation coefficient ≤ -0.990
______________3.920 ≤ slope ≤ -3.103

Bacterial Contamination: Ct ≥ 28

*Functional test -Amplification of a 582bp human β-globin region from dilutions of human genomic DNA standard and no-template control

Key Characteristics

• • Effective for GC rich targets
  • Long target amplification (~2kb)
  • High specificity
  • Effective amplification from crude samples
  • Compatibility with various real-time cyclers
  • Can be used for library quantification

Asia (select countries), Europe, USA