- KOD DNA polymerase exhibits an excellent elongation capability (approx. 130 bases/sec) and strong 3’→5’ exonuclease activity (proofreading activity). However, its sensitivity and efficiency are subject to interference from the enzyme or template concentration during PCR, due to its strong proofreading activity. KOD exo(-) is a 3’→5’ exonuclease minus mutant developed based on KOD DNA polymerase from a hyperthermophilic Archaeon Thermococcus kodakaraensis.
- Concentration/Activity*: 2.5 U/μL
- Endonuclease Activity: None detected
- Nicking Activity: None detected
*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 min at 75°C.
Hotstart Antibody Specification
- Source: Mouse Hybridoma
- Inhibition Activity**: ≥70%
- ssEndonuclease Activity: None detected
- dsEndonuclease Activity: None detected
- 5’ to 3’ Exonuclease Activity: None detected
- 3’ to 5’ Exonuclease Activity: None detected
- Mouse Genomic DNA Contamination: None detected
**Percentage (%) of DNA polymerase activity surpressed when 1.0 μg of antibody is added
to 1.25 units of KOD DNA polymerase at 37°C.
- Excellent processivity & extension ability: Extention time of the thermal cycle can be set <30 seconds / kb.
- Great sensitivity & efficiency: PCR sensitivity and efficiency are improved due to elimination of 3’-5’ exonuclease activity.
- Hot start PCR: anti-KOD DNA polymerase antibody for the polymerase activity is available.
- Can be applied to the real-time PCR: This enzyme can be applied to the real-time PCR assay, except for TaqMan assay due to lacking of the 5’-3’ exonuclease activity. A fast mode cycle can be applied.
Product Availability: Worldwide
Origin: Escherichia coli (R)
Safety Data Sheets
Contact us to get more information, become part of our distribution network, or ask a question about our products and their availability in your region:
|KOD exo(-) DNA Polymerase 5KU
||2mL KOD exo(-) DNA Polymerase|
|KOD exo(-) DNA Polymerase Kit
||0.4mL (1,000U) KOD exo(-) DNA Polymerase, 4mL 10x PCR Buffer for KOD exo(-)|
|10x PCR Buffer for KOD exo(-)
||20mL 10x PCR Buffer for KOD exo(-)|
|Anti-KOD Hotstart Antibody 8.2mg Kit
||8.2mg Anti-KOD,Antibody, Dilution Buffer|