KOD exo(-) DNA Polymerase /Anti-KOD Hotstart Antibody

EC Number

  • # 2 . 7 . 7 . 7


  • KOD DNA polymerase exhibits an excellent elongation capability (approx. 130 bases/sec) and strong 3’→5’ exonuclease activity (proofreading activity). However, its sensitivity and efficiency are subject to interference from the enzyme or template concentration during PCR, due to its strong proofreading activity. KOD exo(-) is a 3’→5’ exonuclease minus mutant developed based on KOD DNA polymerase from a hyperthermophilic Archaeon Thermococcus kodakaraensis.

 Key Specifications

Enzyme Specification

  • Concentration/Activity*: 2.5 U/μL
  • Endonuclease Activity: None detected
  • Nicking Activity: None detected

*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 min at 75°C.

Hotstart Antibody Specification

  • Source: Mouse Hybridoma
  • Inhibition Activity**: ≥70%
  • ssEndonuclease Activity: None detected
  • dsEndonuclease Activity: None detected
  • 5’ to 3’ Exonuclease Activity: None detected
  • 3’ to 5’ Exonuclease Activity: None detected
  • Mouse Genomic DNA Contamination: None detected

**Percentage (%) of DNA polymerase activity surpressed when 1.0 μg of antibody is added

to 1.25 units of KOD DNA polymerase at 37°C.

Key Characteristics

  • Excellent processivity & extension ability: Extention time of the thermal cycle can be set <30 seconds / kb.
  •  Great sensitivity & efficiency: PCR sensitivity and efficiency are improved due to elimination of 3’-5’ exonuclease activity.
  • Hot start PCR: anti-KOD DNA polymerase antibody for the polymerase activity is available.
  • Can be applied to the real-time PCR: This enzyme can be applied to the real-time PCR assay, except for TaqMan assay due to lacking of the 5’-3’ exonuclease activity. A fast mode cycle can be applied.

Product Availability: Worldwide

Origin: Escherichia coli (R)

Safety Data Sheets

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Name Part Size
KOD exo(-) DNA Polymerase 5KU KODND-119 2mL KOD exo(-) DNA Polymerase
KOD exo(-) DNA Polymerase Kit KODND-109B 0.4mL (1,000U) KOD exo(-) DNA Polymerase, 4mL 10x PCR Buffer for KOD exo(-)
10x PCR Buffer for KOD exo(-) KODND-1B 20mL 10x PCR Buffer for KOD exo(-)
Anti-KOD Hotstart Antibody 8.2mg Kit KAB2-101B 8.2mg Anti-KOD,Antibody, Dilution Buffer